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MCF-7 cells probed with SIRT1 Polyclonal Antibody, unconjugated (bs-0921R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
MCF-7 cells probed with SIRT1 Polyclonal Antibody, unconjugated (bs-0921R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
MCF-7 cells probed with SIRT1 Polyclonal Antibody, unconjugated (bs-0921R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

SIRT1 Polyclonal Antibody

BS-0921R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSIRT1
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Overview

  • Supplier
    Bioss
  • Product Name
    SIRT1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID23411
  • Target name
    SIRT1
  • Target description
    sirtuin 1
  • Target synonyms
    SIR2, SIR2L1, SIR2alpha, NAD-dependent protein deacetylase sirtuin-1, NAD-dependent protein deacylase sirtuin-1, SIR2-like protein 1, regulatory protein SIR2 homolog 1, sirtuin type 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96EB6
  • Protein Name
    NAD-dependent protein deacetylase sirtuin-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Protective effect of astaxanthin against contrast-induced acute kidney injury via SIRT1-p53 pathway in rats. Gao D et al., 2019 Feb, Int Urol Nephrol
    Read more
  • Hypothermic machine perfusion ameliorates inflammation during ischemia-reperfusion injury via sirtuin-1-mediated deacetylation of nuclear factor-kappaB p65 in rat livers donated after circulatory death. Zeng C et al., 2017 Dec, Mol Med Rep
    Read more
  • Effects of Aluminium on Long-Term Memory in Rats and on SIRT1 Mediating the Transcription of CREB-Dependent Gene in Hippocampus. Yan D et al., 2017 Oct, Basic Clin Pharmacol Toxicol
    Read more
  • 5-Aminolevulinic acid-mediated sonodynamic therapy induces anti-tumor effects in malignant melanoma via p53-miR-34a-Sirt1 axis. Hu Z et al., 2015 Aug, J Dermatol Sci
    Read more
  • Vam3, a derivative of resveratrol, attenuates cigarette smoke-induced autophagy. Shi J et al., 2012 Jul, Acta Pharmacol Sin
    Read more

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Figure 1. Western blot analysis of SIRT1 using anti-SIRT1 antibody (A00018-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 antigen affinity purified polyclonal antibody (Catalog # A00018-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT1 at approximately 120 kDa. The expected band size for SIRT1 is at 82 kDa.
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