![Wild-type (WT) and SIRT3 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody [C3], C-term (GTX106292) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and SIRT3 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody [C3], C-term (GTX106292) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX106292/GTX106292_42172_20200110_WB_KO_watermark_w_23060120_599.webp)
Wild-type (WT) and SIRT3 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody [C3], C-term (GTX106292) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SIRT3 antibody [C3], C-term
GTX106292
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSIRT3
Overview
- SupplierGeneTex
- Product NameSIRT3 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.7 mg/ml
- ConjugateUnconjugated
- Gene ID23410
- Target nameSIRT3
- Target descriptionsirtuin 3
- Target synonymsSIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, NAD-dependent deacetylase sirtuin-3, mitochondrial, NAD-dependent protein delactylase sirtuin-3, SIR2-like protein 3, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, regulatory protein SIR2 homolog 3, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, sirtuin type 3
- HostRabbit
- IsotypeIgG
- Protein IDQ9NTG7
- Protein NameNAD-dependent protein deacetylase sirtuin-3, mitochondrial
- Scientific DescriptionThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chen IC, Chiang WF, Chen PF, et al. STRESS-responsive deacetylase SIRT3 is up-regulated by areca nut extract-induced oxidative stress in human oral keratinocytes. J Cell Biochem. 2014,115(2):328-39. doi: 10.1002/jcb.24667Read this paper



![SIRT3 antibody [C3], C-term detects SIRT3 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. SIRT3 antibody [C3], C-term (GTX106292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min SIRT3 antibody [C3], C-term detects SIRT3 protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. SIRT3 antibody [C3], C-term (GTX106292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX106292/GTX106292_42172_20151013_IHC-P_w_23060120_672.webp)





