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WB analysis of human kidney lysate using GTX10140 SIRT4 antibody. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human kidney lysate using GTX10140 SIRT4 antibody. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human kidney lysate using GTX10140 SIRT4 antibody. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer

SIRT4 antibody

GTX10140
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSIRT4
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Overview

  • Supplier
    GeneTex
  • Product Name
    SIRT4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-3microg/ml. IHC-P: 2-3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23409
  • Target name
    SIRT4
  • Target description
    sirtuin 4
  • Target synonyms
    NAD-dependent ADP-ribosyltransferase sirtuin-4; NAD-dependent protein deacetylase sirtuin-4; NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; regulatory protein SIR2 homolog 4; SIR2L4; sir2-like 4; SIR2-like protein 4; sirtuin type 4
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9Y6E7
  • Protein Name
    NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
  • Scientific Description
    This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SIRT4 using anti-SIRT4 antibody (A03764-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT4 antigen affinity purified polyclonal antibody (Catalog # A03764-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT4 at approximately 38KD. The expected band size for SIRT4 is at 35KD.
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