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SIRT4 Antibody

MBS8517615

Product group Antibodies

Overview

Supplier
MyBioSource
Product Name
SIRT4 Antibody
Delivery Days Customer
16
Certification
Research Use Only
UNSPSC
12352203

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Related products

Product group Antibodies

Anti-SIRT4 Antibody144-07585

ApplicationsWestern Blot

ReactivityHuman, Mouse, Rat

TargetSIRT4

SizePrice

Figure 1. Western blot analysis of SIRT4 using anti-SIRT4 antibody (A03764-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT4 antigen affinity purified polyclonal antibody (Catalog # A03764-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT4 at approximately 38KD. The expected band size for SIRT4 is at 35KD.

Product group Antibodies

Anti-SIRT4 Antibody Picoband(r)A03764-1-CARRIER-FREE

ApplicationsWestern Blot, ImmunoHistoChemistry

ReactivityHuman, Mouse, Rat

TargetSIRT4

SizePrice

SIRT4 antibody detects SIRT4 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SIRT4 antibody (GTX129978) diluted by 1:1000.

Product group Antibodies

SIRT4 antibodyGTX129978

ApplicationsWestern Blot

ReactivityHuman

TargetSIRT4

SizePrice

Product group Antibodies

Sirtuin 4 (SIRT4) Polyclonal AntibodyCAU22622

ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry

TargetSIRT4

SizePrice

$Paraformaldehyde-fixed, paraffin embedded rat stomach tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-SIRT4 Polyclonal Antibody, Unconjugated (bs-7537R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining\n$