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WB analysis of various samples using GTX01549 SIRT6 antibody. Lane 1 : HeLa whole cell lysate at 40μg Lane 2 : A549 whole cell lysate at 40μg Lane 3 : human placenta tissue lysate at 50μg Dilution : 0.5μg/ml
WB analysis of various samples using GTX01549 SIRT6 antibody. Lane 1 : HeLa whole cell lysate at 40μg Lane 2 : A549 whole cell lysate at 40μg Lane 3 : human placenta tissue lysate at 50μg Dilution : 0.5μg/ml
WB analysis of various samples using GTX01549 SIRT6 antibody. Lane 1 : HeLa whole cell lysate at 40μg Lane 2 : A549 whole cell lysate at 40μg Lane 3 : human placenta tissue lysate at 50μg Dilution : 0.5μg/ml

SIRT6 antibody

GTX01549
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSIRT6
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Overview

  • Supplier
    GeneTex
  • Product Name
    SIRT6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. IHC-P: 0.5-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51548
  • Target name
    SIRT6
  • Target description
    sirtuin 6
  • Target synonyms
    SIR2L6, hSIRT6, NAD-dependent protein deacylase sirtuin-6, NAD-dependent protein deacetylase sirtuin-6, SIR2-like protein 6, protein mono-ADP-ribosyltransferase sirtuin-6, regulatory protein SIR2 homolog 6, sir2-related protein type 6, sirtuin type 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N6T7
  • Protein Name
    NAD-dependent protein deacylase sirtuin-6
  • Scientific Description
    This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SIRT6 using anti-SIRT6 antibody (A00611-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT6 antigen affinity purified polyclonal antibody (Catalog # A00611-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT6 at approximately 45 kDa. The expected band size for SIRT6 is at 37 kDa.
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