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SIRT7 / Sirtuin 7 Antibody

LS-C404282
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSIRT7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SIRT7 / Sirtuin 7 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51547
  • Target name
    SIRT7
  • Target description
    sirtuin 7
  • Target synonyms
    SIR2L7, NAD-dependent protein deacetylase sirtuin-7, NAD-dependent deacetylase sirtuin-7, NAD-dependent protein deacylase sirtuin-7, SIR2-like protein 7, regulatory protein SIR2 homolog 7, silent mating type information regulation 2, S.cerevisiae, homolog 7, sir2-related protein type 7, sirtuin type 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SIRT7 using anti-SIRT7 antibody (PB9358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thyums tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT7 antigen affinity purified polyclonal antibody (Catalog # PB9358) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT7 at approximately 40 kDa. The expected band size for SIRT7 is at 45 kDa.
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