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SIX3 Antibody (Internal)

LS-C352840
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSIX3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SIX3 Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6496
  • Target name
    SIX3
  • Target description
    SIX homeobox 3
  • Target synonyms
    homeobox protein SIX3; HPE2; sine oculis homeobox homolog 3; sine oculis homeobox-like protein 3; truncated SIX3
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Six3 using anti-Six3 antibody (A02604). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: mouse brain tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Six3 antigen affinity purified polyclonal antibody (Catalog # A02604) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Six3 at approximately 35KD. The expected band size for Six3 is at 35KD.
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