WB analysis of human testis tissue lysate using GTX85013 SKA3 antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml
WB analysis of human testis tissue lysate using GTX85013 SKA3 antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml
SKA3 antibody
GTX85013
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSKA3
Overview
- SupplierGeneTex
- Product NameSKA3 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 0.5 - 1 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID221150
- Target nameSKA3
- Target descriptionspindle and kinetochore associated complex subunit 3
- Target synonymsC13orf3; RAMA1; spindle and kinetochore-associated protein 3
- HostRabbit
- IsotypeIgG
- Protein IDQ8IX90
- Protein NameSpindle and kinetochore-associated protein 3
- Scientific DescriptionUpon entry into mitosis, the cells microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.
- ReactivityHuman, Mouse, Rat
- Storage Instruction2°C to 8°C
- UNSPSC12352203