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SKAP1 / SCAP1 Antibody (AP)

LS-C408696
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSKAP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SKAP1 / SCAP1 Antibody (AP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 41kDa, while the observed MW by Western blot was 55kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:20 - 1:200), WB (1:200 - 1:2000) The predicted MW is 41kDa, while the observed MW by Western blot was 55kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Alk. Phosphatase
  • Estimated Purity
    ...
  • Gene ID8631
  • Target name
    SKAP1
  • Target description
    src kinase associated phosphoprotein 1
  • Target synonyms
    HEL-S-81p, SCAP1, SKAP55, src kinase-associated phosphoprotein 1, SKAP-55, epididymis secretory sperm binding protein Li 81p, pp55, src family-associated phosphoprotein 1, src kinase-associated phosphoprotein of 55 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SKAP55 using anti-SKAP55 antibody (PB9890). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: PANC whole cell lysates, Lane 2: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKAP55 antigen affinity purified polyclonal antibody (Catalog # PB9890) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKAP55 at approximately 60 kDa. The expected band size for SKAP55 is at 41 kDa.
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