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SKAP2 / SCAP2 Antibody (aa137-165)

LS-C325131
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSKAP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SKAP2 / SCAP2 Antibody (aa137-165)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:1000), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8935
  • Target name
    SKAP2
  • Target description
    src kinase associated phosphoprotein 2
  • Target synonyms
    Fyn-associated phosphoprotein SKAP55 homologue; PRAP; Pyk2/RAFTK-associated protein; RA70; retinoic acid-induced protein 70; SAPS; SCAP2; SKAP55 homolog; SKAP-55HOM; SKAP55R; SKAP-HOM; src family-associated phosphoprotein 2; src kinase-associated phosphoprotein 2; src kinase-associated phosphoprotein of 55-related protein; src-associated adapter protein with PH and SH3 domains
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SKAP2 using anti-SKAP2 antibody (A06280-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKAP2 antigen affinity purified polyclonal antibody (Catalog # A06280-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKAP2 at approximately 41 kDa. The expected band size for SKAP2 is at 41 kDa.
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