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Western Blot analysis of Jurkat cells using SnoN Polyclonal Antibody
Western Blot analysis of Jurkat cells using SnoN Polyclonal Antibody
Western Blot analysis of Jurkat cells using SnoN Polyclonal Antibody

SKIL Antibody

CSB-PA004125
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSKIL
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Overview

  • Supplier
    Cusabio
  • Product Name
    SKIL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6498
  • Target name
    SKIL
  • Target description
    SKI like proto-oncogene
  • Target synonyms
    SKI-like oncogene; ski-like protein; ski-related oncogene snoN; SNO; SnoA; SnoI; SnoN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12757
  • Protein Name
    Ski-like protein
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SnoN/SNO/SKIL using anti-SnoN/SNO/SKIL antibody (A04131-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Sw620 whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SnoN/SNO/SKIL antigen affinity purified polyclonal antibody (Catalog # A04131-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SnoN/SNO/SKIL at approximately 77 kDa. The expected band size for SnoN/SNO/SKIL is at 77 kDa.
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