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ProductsBack/SLAMF6 / NTBA Antibody (clone 040, Preservative Free)

SLAMF6 / NTBA Antibody (clone 040, Preservative Free)

LS-C819367
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSLAMF6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLAMF6 / NTBA Antibody (clone 040, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    040
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID114836
  • Target name
    SLAMF6
  • Target description
    SLAM family member 6
  • Target synonyms
    CD352, KALI, KALIb, Ly108, NTB-A, NTBA, SF2000, SLAM family member 6, NK-T-B-antigen, NTBA receptor, activating NK receptor, natural killer-, T- and B-cell antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLAMF6 using anti-SLAMF6 antibody (A05310-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLAMF6 antigen affinity purified polyclonal antibody (Catalog # A05310-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLAMF6 at approximately 60 kDa. The expected band size for SLAMF6 is at 37 kDa.
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