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Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: SLC12A2 antibody at 6.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 132, 130 kDa Observed band size: 132 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: SLC12A2 antibody at 6.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 132, 130 kDa Observed band size: 132 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Mouse kidney tissue All lanes: SLC12A2 antibody at 6.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 132, 130 kDa Observed band size: 132 kDa

SLC12A2 Antibody

CSB-PA021383LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC12A2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC12A2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6558
  • Target name
    SLC12A2
  • Target description
    solute carrier family 12 member 2
  • Target synonyms
    basolateral Na-K-Cl symporter; BSC; BSC2; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; KILQS; NKCC1; PPP1R141; protein phosphatase 1, regulatory subunit 141; solute carrier family 12 (sodium/potassium/chloride transporter), member 2; solute carrier family 12 (sodium/potassium/chloride transporters), member 2; solute carrier family 12 member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55011
  • Protein Name
    Solute carrier family 12 member 2
  • Scientific Description
    Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (A03603). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKCC1/SLC12A2 antigen affinity purified polyclonal antibody (Catalog # A03603) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NKCC1/SLC12A2 at approximately 180 kDa. The expected band size for NKCC1/SLC12A2 is at 180 kDa.
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