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SLC12A6 / KCC3 Antibody

LS-C402540
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSLC12A6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC12A6 / KCC3 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9990
  • Target name
    SLC12A6
  • Target description
    solute carrier family 12 member 6
  • Target synonyms
    ACCPN, CMT2II, KCC3, KCC3A, KCC3B, solute carrier family 12 member 6, K-Cl cotransporter 3, electroneutral potassium-chloride cotransporter 3, potassium chloride cotransporter 3, potassium chloride cotransporter KCC3a-S3, potassium-chloride transporter-3a, potassium-chloride transporter-3b, solute carrier family 12 (potassium/chloride transporter), member 6, solute carrier family 12 (potassium/chloride transporters), member 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC12A6 using anti-SLC12A6 antibody (PA2171). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC12A6 antigen affinity purified polyclonal antibody (Catalog # PA2171) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC12A6 at approximately 128 kDa. The expected band size for SLC12A6 is at 128 kDa.
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