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Western Blot Positive WB detected in: Mouse liver tissue All lanes: SLC18A1 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 51, 53 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: Mouse liver tissue All lanes: SLC18A1 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 51, 53 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: Mouse liver tissue All lanes: SLC18A1 antibody at 2.7ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 51, 53 kDa Observed band size: 57 kDa

SLC18A1 Antibody

CSB-PA021426LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC18A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC18A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6570
  • Target name
    SLC18A1
  • Target description
    solute carrier family 18 member A1
  • Target synonyms
    CGAT, VAT1, VMAT1, chromaffin granule amine transporter, solute carrier family 18 (vesicular monoamine transporter), member 1, solute carrier family 18 (vesicular monoamine), member 1, solute carrier family 18 member 1, vesicular amine transporter 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54219
  • Protein Name
    Chromaffin granule amine transporter
  • Scientific Description
    Involved in the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles of neuroendocrine and endocrine cells.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC18A1 using anti-SLC18A1 antibody (A07692-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human HL-60 cell lysates, Lane 6: human K562 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC18A1 antigen affinity purified polyclonal antibody (Catalog # A07692-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC18A1 at approximately 56KD. The expected band size for SLC18A1 is at 56KD.
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