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ProductsBack/SLC1A2 / EAAT2 / GLT-1 Antibody (aa495-574)

SLC1A2 / EAAT2 / GLT-1 Antibody (aa495-574)

LS-C408264
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSLC1A2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC1A2 / EAAT2 / GLT-1 Antibody (aa495-574)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 61kDa/62kDa, while the observed MW by Western blot was 65kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB (1:500 - 1:5000) The predicted MW is 61kDa/62kDa, while the observed MW by Western blot was 65kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6506
  • Target name
    SLC1A2
  • Target description
    solute carrier family 1 member 2
  • Target synonyms
    DEE41, EAAT2, EIEE41, GLT-1, GLT1, HBGT, excitatory amino acid transporter 2, excitotoxic amino acid transporter 2, glutamate transporter-1, glutamate/aspartate transporter II, human brain glutamate transporter, sodium-dependent glutamate/aspartate transporter 2, solute carrier family 1 (glial high affinity glutamate transporter), member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EAAT2/GLT-1/SLC1A2 using anti-EAAT2/GLT-1/SLC1A2 antibody (RP1065). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT2/GLT-1/SLC1A2 antigen affinity purified polyclonal antibody (Catalog # RP1065) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT2/GLT-1/SLC1A2 at approximately 65 kDa. The expected band size for EAAT2/GLT-1/SLC1A2 is at 62 kDa.
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