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Western Blot analysis of HeLa cells using EAAT1 Polyclonal Antibody
Western Blot analysis of HeLa cells using EAAT1 Polyclonal Antibody
Western Blot analysis of HeLa cells using EAAT1 Polyclonal Antibody

SLC1A3 Antibody

CSB-PA002240
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSLC1A3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC1A3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6507
  • Target name
    SLC1A3
  • Target description
    solute carrier family 1 member 3
  • Target synonyms
    EA6; EAAT1; excitatory amino acid transporter 1; GLAST; GLAST1; GLAST-1; sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43003
  • Protein Name
    Excitatory amino acid transporter 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (A02133-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT1/SLC1A3 antigen affinity purified polyclonal antibody (Catalog # A02133-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT1/SLC1A3 at approximately 55 kDa. The expected band size for EAAT1/SLC1A3 is at 55 kDa.
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