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ProductsBack/SLC1A3 / EAAT1 Antibody (clone D20D5)

SLC1A3 / EAAT1 Antibody (clone D20D5)

LS-C183890
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetSLC1A3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC1A3 / EAAT1 Antibody (clone D20D5)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP, WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    D20D5
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6507
  • Target name
    SLC1A3
  • Target description
    solute carrier family 1 member 3
  • Target synonyms
    EA6; EAAT1; excitatory amino acid transporter 1; GLAST; GLAST1; GLAST-1; sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (A02133-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT1/SLC1A3 antigen affinity purified polyclonal antibody (Catalog # A02133-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT1/SLC1A3 at approximately 55 kDa. The expected band size for EAAT1/SLC1A3 is at 55 kDa.
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