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ProductsBack/SLC20A1 Antibody (Cytoplasmic Domain)

SLC20A1 Antibody (Cytoplasmic Domain)

LS-C17283
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSLC20A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC20A1 Antibody (Cytoplasmic Domain)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Suitable for use in Western Blot and ELISA. Western Blot: 1-10 ug/ml (ECL). A band of 85-90kD has been detected in brush border membrane. Additional bands of 70-72kD may represent proteolytic fragments of PiT-1. ELISA: 0.5-1 ug/ml; Control peptide can be used to coat ELISA plates at 1 ug/ml.. ELISA (0.5 - 1 µg/ml), WB (1 - 10 µg/ml) Suitable for use in Western Blot and ELISA. Western Blot: 1-10 ug/ml (ECL). A band of 85-90kD has been detected in brush border membrane. Additional bands of 70-72kD may represent proteolytic fragments of PiT-1. ELISA: 0.5-1 ug/ml; Control peptide can be used to coat ELISA plates at 1 ug/ml.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6574
  • Target name
    SLC20A1
  • Target description
    solute carrier family 20 member 1
  • Target synonyms
    gibbon ape leukemia virus receptor 1; GLVR1; Glvr-1; leukemia virus receptor 1 homolog; PIT1; PiT-1; sodium-dependent phosphate transporter 1; solute carrier family 20 (phosphate transporter), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC20A1 antigen affinity purified polyclonal antibody (Catalog # A03537-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC20A1 at approximately 85 kDa. The expected band size for SLC20A1 is at 74 kDa.
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