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Western Blot analysis of HuvEc, K562 cells using OAT1 Polyclonal Antibody.
Western Blot analysis of HuvEc, K562 cells using OAT1 Polyclonal Antibody.
Western Blot analysis of HuvEc, K562 cells using OAT1 Polyclonal Antibody.

SLC22A6 Antibody

CSB-PA005620
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSLC22A6
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC22A6 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9356
  • Target name
    SLC22A6
  • Target description
    solute carrier family 22 member 6
  • Target synonyms
    HOAT1; hPAHT; hROAT1; OAT1; organic anion transporter 1; PAH transporter; PAHT; para-aminohippurate transporter; renal organic anion transporter 1; ROAT1; solute carrier family 22 (organic anion transporter), member 6; solute carrier family 22 member 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ4U2R8
  • Protein Name
    Solute carrier family 22 member 6
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OAT1/SLC22A6 using anti-OAT1/SLC22A6 antibody (A02456-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT1/SLC22A6 antigen affinity purified polyclonal antibody (Catalog # A02456-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT1/SLC22A6 at approximately 70 kDa. The expected band size for OAT1/SLC22A6 is at 62 kDa.
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