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SLC25A1 / SEA Antibody

LS-C496956
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC25A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC25A1 / SEA Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 34kDa, while the observed MW by Western blot was 30kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB (1:200 - 1:2000) The predicted MW is 34kDa, while the observed MW by Western blot was 30kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6576
  • Target name
    SLC25A1
  • Target description
    solute carrier family 25 member 1
  • Target synonyms
    CIC, CMS23, CTP, D2L2AD, SEA, SLC20A3, tricarboxylate transport protein, mitochondrial, citrate isocitrate carrier, citrate transport protein, mitochondrial citrate carrier, solute carrier family 20 (mitochondrial citrate transporter), member 3, solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1, tricarboxylate carrier protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of SLC25A1 using anti-SLC25A1 antibody (A05995-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A1 antigen affinity purified polyclonal antibody (Catalog # A05995-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A1 at approximately 30-34KD. The expected band size for SLC25A1 is at 34KD.
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