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ProductsBack/SLC25A13 / CITRIN Antibody

SLC25A13 / CITRIN Antibody

LS-C402028
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSLC25A13
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC25A13 / CITRIN Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:25 - 1:100), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10165
  • Target name
    SLC25A13
  • Target description
    solute carrier family 25 member 13
  • Target synonyms
    ARALAR2, CITRIN, CTLN2, NICCD, electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial, ARALAR-related gene 2, calcium-binding mitochondrial carrier protein Aralar2, citrullinemia type II, mitochondrial aspartate glutamate carrier 2, solute carrier family 25 (aspartate/glutamate carrier), member 13
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Citrin/SLC25A13 using anti-Citrin/SLC25A13 antibody (A03476-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Citrin/SLC25A13 antigen affinity purified polyclonal antibody (Catalog # A03476-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Citrin/SLC25A13 at approximately 70 kDa. The expected band size for Citrin/SLC25A13 is at 74 kDa.
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