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SLC25A19 Antibody

LS-C747483
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSLC25A19
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC25A19 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 29kDa/35kDa, while the observed MW by Western blot was 36kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 29kDa/35kDa, while the observed MW by Western blot was 36kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID60386
  • Target name
    SLC25A19
  • Target description
    solute carrier family 25 member 19
  • Target synonyms
    DNC, MCPHA, MTPPT, MUP1, THMD3, THMD4, TPC, mitochondrial thiamine pyrophosphate carrier, Deoxynucleotide carrier, mitochondrial thiamine pyrophosphate transporter, mitochondrial uncoupling protein 1, solute carrier family 25 (mitochondrial deoxynucleotide carrier), member 19, solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC25A19 using anti-SLC25A19 antibody (A07902-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A19 antigen affinity purified polyclonal antibody (Catalog # A07902-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A19 at approximately 41KD. The expected band size for SLC25A19 is at 41KD.
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