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Western Blot Positive WB detected in: Mouse brain tissue, Mouse kidney tissue All lanes: SLC25A23 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 50, 55, 49 kDa Observed band size: 53 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Mouse kidney tissue All lanes: SLC25A23 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 50, 55, 49 kDa Observed band size: 53 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Mouse kidney tissue All lanes: SLC25A23 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53, 50, 55, 49 kDa Observed band size: 53 kDa

SLC25A23 Antibody

CSB-PA871580LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC25A23
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC25A23 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79085
  • Target name
    SLC25A23
  • Target description
    solute carrier family 25 member 23
  • Target synonyms
    APC2, MCSC2, SCAMC3, SCaMC-3, mitochondrial adenyl nucleotide antiporter SLC25A23, calcium-binding mitochondrial carrier protein SCaMC-3, mitochondrial ATP-Mg/Pi carrier protein 2, mitochondrial Ca(2+)-dependent solute carrier protein 2, mitochondrial Ca2+-dependent solute carrier protein 2, short calcium-binding mitochondrial carrier 3, short calcium-binding mitochondrial carrier protein 3, small calcium-binding mitochondrial carrier protein 3, solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 23
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BV35
  • Protein Name
    Calcium-binding mitochondrial carrier protein SCaMC-3
  • Scientific Description
    Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane (PubMed:15123600). May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria (PubMed:15123600). Acts as a regulator of mitochondrial calcium uptake via interaction with MCU and MICU1 (PubMed:24430870).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC25A23 using anti-SLC25A23 antibody (A08997-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tisue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A23 antigen affinity purified polyclonal antibody (Catalog # A08997-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A23 at approximately 48-54 kDa. The expected band size for SLC25A23 is at 52 kDa.
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