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ProductsBack/SLC25A24 Antibody
Western Blot Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, Rat kidney tissue, Mouse heart tissue All lanes: SLC25A24 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54, 52 kDa Observed band size: 54 kDa
Western Blot Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, Rat kidney tissue, Mouse heart tissue All lanes: SLC25A24 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54, 52 kDa Observed band size: 54 kDa
Western Blot Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, Rat kidney tissue, Mouse heart tissue All lanes: SLC25A24 antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54, 52 kDa Observed band size: 54 kDa

SLC25A24 Antibody

CSB-PA743993LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC25A24
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC25A24 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29957
  • Target name
    SLC25A24
  • Target description
    solute carrier family 25 member 24
  • Target synonyms
    APC1, SCAMC-1, SCAMC1, mitochondrial adenyl nucleotide antiporter SLC25A24, ATP-Mg/P(i) co-transporter 1, calcium-binding mitochondrial carrier protein SCaMC-1, calcium-binding transporter, mitochondrial ATP-Mg/Pi carrier protein 1, mitochondrial Ca(2+)-dependent solute carrier protein 1, short calcium-binding mitochondrial carrier 1, short calcium-binding mitochondrial carrier protein 1, small calcium-binding mitochondrial carrier protein 1, solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 24
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6NUK1
  • Protein Name
    Mitochondrial adenyl nucleotide antiporter SLC25A24
  • Scientific Description
    Calcium-dependent mitochondrial solute carrier. Mediates the reversible, electroneutral exchange of Mg-ATP or Mg-ADP against phosphate ions, catalyzing the net uptake or efflux of adenine nucleotides across the mitochondrial inner membrane. Nucleotide transport is inactive when cytosolic calcium levels are low, and is activated by an increase in cytosolic calcium levels. May play a role in protecting cells against oxidative stress-induced cell death, probably by promoting the formation of calcium-phosphate precipitates in the mitochondrial matrix, and thereby buffering calcium levels in the mitochondrial matrix.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A24 antigen affinity purified polyclonal antibody (Catalog # A08355-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A24 at approximately 45 kDa. The expected band size for SLC25A24 is at 53 kDa.
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