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ProductsBack/SLC25A25 Antibody
Western blot All lanes: SLC25A25 antibody at 10microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 56, 57, 55, 41 kDa Observed band size: 53 kDa
Western blot All lanes: SLC25A25 antibody at 10microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 56, 57, 55, 41 kDa Observed band size: 53 kDa
Western blot All lanes: SLC25A25 antibody at 10microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 56, 57, 55, 41 kDa Observed band size: 53 kDa

SLC25A25 Antibody

CSB-PA760850LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC25A25
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC25A25 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID114789
  • Target name
    SLC25A25
  • Target description
    solute carrier family 25 member 25
  • Target synonyms
    MCSC, PCSCL, SCAMC-2, SCAMC2, mitochondrial adenyl nucleotide antiporter SLC25A25, calcium-binding mitochondrial carrier protein SCaMC-2, mitochondrial ATP-Mg/Pi carrier protein 3, mitochondrial Ca(2+)-dependent solute carrier protein 3, short calcium-binding mitochondrial carrier 2, short calcium-binding mitochondrial carrier protein 2, small calcium-binding mitochondrial carrier 2, solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6KCM7
  • Protein Name
    Mitochondrial adenyl nucleotide antiporter SLC25A25
  • Scientific Description
    Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC25A25 using anti-SLC25A25 antibody (A09109-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A25 antigen affinity purified polyclonal antibody (Catalog # A09109-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A25 at approximately 50-55 kDa. The expected band size for SLC25A25 is at 53 kDa.
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