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Immunohistochemistry of paraffin-embedded Human colon cancer tissue using SLC2A2 Polyclonal Antibody at dilution 1:30
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using SLC2A2 Polyclonal Antibody at dilution 1:30
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using SLC2A2 Polyclonal Antibody at dilution 1:30

SLC2A2 Polyclonal Antibody

E-AB-15690
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SLC2A2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. The encoded protein mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. Mutations in this gene are associated with susceptibility to diseases, including Fanconi-Bickel syndrome and noninsulin-dependent diabetes mellitus (NIDDM). Alternative splicing results in multiple transcript variants of this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC2A2 using anti-SLC2A2 antibody (PB9436). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: PANC Whole Cell Lysate at 40ug, Lane 2: A549 Whole Cell Lysate at 40ug, Lane 3: HT1080 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A2 antigen affinity purified polyclonal antibody (Catalog # PB9436) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A2 at approximately 50 kDa. The expected band size for SLC2A2 is at 57 kDa.
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