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Western Blot analysis of K562 cells using Glut5 Polyclonal Antibody.
Western Blot analysis of K562 cells using Glut5 Polyclonal Antibody.
Western Blot analysis of K562 cells using Glut5 Polyclonal Antibody.

SLC2A5 Antibody

CSB-PA005624
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSLC2A5
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC2A5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6518
  • Target name
    SLC2A5
  • Target description
    solute carrier family 2 member 5
  • Target synonyms
    glucose transporter type 5, small intestine; glucose transporter-like protein 5; GLUT5; GLUT-5; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; solute carrier family 2, facilitated glucose transporter member 5; testicular tissue protein Li 81
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22732
  • Protein Name
    Solute carrier family 2, facilitated glucose transporter member 5
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC2A5 using anti-SLC2A5 antibody (PB9960). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A5 antigen affinity purified polyclonal antibody (Catalog # PB9960) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A5 at approximately 55KD. The expected band size for SLC2A5 is at 55KD.
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