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ProductsBack/SLC2A6 Mouse Monoclonal Antibody [Clone ID: OTI7E3]
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-SLC2A6mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-SLC2A6mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-SLC2A6mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

SLC2A6 Mouse Monoclonal Antibody [Clone ID: OTI7E3]

CF500639
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC2A6
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SLC2A6 Mouse Monoclonal Antibody [Clone ID: OTI7E3]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI7E3
  • Gene ID11182
  • Target name
    SLC2A6
  • Target description
    solute carrier family 2 member 6
  • Target synonyms
    GLUT6, GLUT9, HSA011372, solute carrier family 2, facilitated glucose transporter member 6, GLUT-6, GLUT-9, glucose transporter type 6, glucose transporter type 9, solute carrier family 2 (facilitated glucose transporter), member 6
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ9UGQ3
  • Protein Name
    Solute carrier family 2, facilitated glucose transporter member 6
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SLC2A6 mouse monoclonal antibody, clone OTI7E3 (formerly 7E3)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC2A6 using anti-SLC2A6 antibody (A11862-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A6 antigen affinity purified polyclonal antibody (Catalog # A11862-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A6 at approximately 55-60KD. The expected band size for SLC2A6 is at 55KD.
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