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ProductsBack/SLC2A9 / GLUT9 Antibody

SLC2A9 / GLUT9 Antibody

LS-C749594
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC2A9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC2A9 / GLUT9 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 55kDa/58kDa, while the observed MW by Western blot was 60kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 55kDa/58kDa, while the observed MW by Western blot was 60kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID56606
  • Target name
    SLC2A9
  • Target description
    solute carrier family 2 member 9
  • Target synonyms
    GLUT9, GLUTX, UAQTL2, URATv1, solute carrier family 2, facilitated glucose transporter member 9, GLUT-9, glucose transporter type 9, human glucose transporter-like protein-9, solute carrier family 2 (facilitated glucose transporter), member 9, urate transporter, urate voltage-driven efflux transporter 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC2A9 using anti-SLC2A9 antibody (A02245). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: rat kidney tissue lysates, Lane 8: rat liver tissue lysates, Lane 9: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A9 antigen affinity purified polyclonal antibody (Catalog # A02245) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A9 at approximately 59KD. The expected band size for SLC2A9 is at 59KD.
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