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ProductsBack/SLC37A3 Antibody (aa224-273)

SLC37A3 Antibody (aa224-273)

LS-C40445
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSLC37A3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC37A3 Antibody (aa224-273)
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P, WB (0.2 - 1 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84255
  • Target name
    SLC37A3
  • Target description
    solute carrier family 37 member 3
  • Target synonyms
    SPX3, sugar phosphate exchanger 3, solute carrier family 37 (glycerol-3-phosphate transporter), member 3, sugar-phosphate exchange protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC37A3 using anti-SLC37A3 antibody (A14532-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC37A3 antigen affinity purified polyclonal antibody (Catalog # A14532-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC37A3 at approximately 50 kDa. The expected band size for SLC37A3 is at 54 kDa.
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