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Western Blot analysis of COLO205 cells using ZIP7 Polyclonal Antibody
Western Blot analysis of COLO205 cells using ZIP7 Polyclonal Antibody
Western Blot analysis of COLO205 cells using ZIP7 Polyclonal Antibody

SLC39A7 Antibody

CSB-PA004569
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC39A7
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC39A7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7922
  • Target name
    SLC39A7
  • Target description
    solute carrier family 39 member 7
  • Target synonyms
    AGM9, D6S115E, D6S2244E, H2-KE4, HKE4, KE4, RING5, ZIP7, zinc transporter SLC39A7, HLA class II region expressed gene KE4, Ke4 gene, mouse, human homolog of, histidine-rich membrane protein Ke4, really interesting new gene 5 protein, solute carrier family 39 (zinc transporter), member 7, zinc transporter 7, zrt-, Irt-like protein 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92504
  • Protein Name
    Zinc transporter SLC39A7
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of ZIP7/SLC39A7 using anti-ZIP7/SLC39A7 antibody (A07719-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIP7/SLC39A7 antigen affinity purified polyclonal antibody (Catalog # A07719-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZIP7/SLC39A7 at approximately 50 kDa. The expected band size for ZIP7/SLC39A7 is at 50 kDa.
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