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WB analysis of rat liver tissue lysate using GTX66266 SLC47A1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of rat liver tissue lysate using GTX66266 SLC47A1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of rat liver tissue lysate using GTX66266 SLC47A1 antibody. Dilution : 1:1000 Loading : 25microg per lane

SLC47A1 antibody

GTX66266
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSLC47A1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SLC47A1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55244
  • Target name
    SLC47A1
  • Target description
    solute carrier family 47 member 1
  • Target synonyms
    MATE1, multidrug and toxin extrusion protein 1, MATE-1, hMATE-1, multidrug and toxin extrusion 1, solute carrier family 47 (multidrug and toxin extrusion), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96FL8
  • Protein Name
    Multidrug and toxin extrusion protein 1
  • Scientific Description
    This gene is located within the Smith-Magenis syndrome region on chromosome 17. It encodes a protein of unknown function. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Nishizawa K, Yoda N, Morokado F, et al. Changes of drug pharmacokinetics mediated by downregulation of kidney organic cation transporters Mate1 and Oct2 in a rat model of hyperuricemia. PLoS One. 2019,14(4):e0214862. doi: 10.1371/journal.pone.0214862
    Read this paper

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Figure 1. Western blot analysis of MATE-1/SLC47A1 using anti-MATE-1/SLC47A1 antibody (A04529-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATE-1/SLC47A1 antigen affinity purified polyclonal antibody (Catalog # A04529-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MATE-1/SLC47A1 at approximately 32 kDa. The expected band size for MATE-1/SLC47A1 is at 62 kDa.
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