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Western Blot Positive WB detected in: Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue All lanes: SLC5A1 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 61 kDa Observed band size: 74 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue All lanes: SLC5A1 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 61 kDa Observed band size: 74 kDa
Western Blot Positive WB detected in: Mouse heart tissue, Mouse kidney tissue, Mouse brain tissue All lanes: SLC5A1 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 74, 61 kDa Observed band size: 74 kDa

SLC5A1 Antibody

CSB-PA021675LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC5A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC5A1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6523
  • Target name
    SLC5A1
  • Target description
    solute carrier family 5 member 1
  • Target synonyms
    D22S675, NAGT, SGLT-1, SGLT1, sodium/glucose cotransporter 1, Na+/glucose cotransporter 1, high affinity sodium-glucose cotransporter, solute carrier family 5 (sodium/glucose cotransporter), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13866
  • Protein Name
    Sodium/glucose cotransporter 1
  • Scientific Description
    Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of SLC5A1 using anti-SLC5A1 antibody (PA2244). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat NRK whole cell lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A1 antigen affinity purified polyclonal antibody (Catalog # PA2244) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A1 at approximately 73 kDa. The expected band size for SLC5A1 is at 73 kDa.
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