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Immunofluorescence staining of HepG2 cells with CSB-PA23589A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cells with CSB-PA23589A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cells with CSB-PA23589A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SLC5A4 Antibody

CSB-PA23589A0RB
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetSLC5A4
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC5A4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6527
  • Target name
    SLC5A4
  • Target description
    solute carrier family 5 member 4
  • Target synonyms
    DJ90G24.4; low affinity sodium-glucose cotransporter; Na(+)/glucose cotransporter 3; SAAT1; SGLT3; sodium transporter; sodium/glucose cotransporter 3; solute carrier family 5 (glucose activated ion channel), member 4; solute carrier family 5 (low affinity glucose cotransporter), member 4; solute carrier family 5 (neutral amino acid transporters, system A), member 4; solute carrier family 5 member 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NY91
  • Protein Name
    Solute carrier family 5 member 4
  • Scientific Description
    Sodium-dependent glucose transporter.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC5A4 using anti-SLC5A4 antibody (A15467-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A4 antigen affinity purified polyclonal antibody (Catalog # A15467-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A4 at approximately 80 kDa. The expected band size for SLC5A4 is at 72 kDa.
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