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ProductsBack/SLC5A8 antibody [HL2687]

SLC5A8 antibody [HL2687]

GTX639352
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSLC5A8
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Overview

  • Supplier
    GeneTex
  • Product Name
    SLC5A8 antibody [HL2687]
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2687
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID160728
  • Target name
    SLC5A8
  • Target description
    solute carrier family 5 member 8
  • Target synonyms
    AIT, SMCT, SMCT1, sodium-coupled monocarboxylate transporter 1, apical iodide transporter, electrogenic sodium monocarboxylate cotransporter, sodium iodide-related cotransporter, solute carrier family 5 (iodide transporter), member 8, solute carrier family 5 (sodium/monocarboxylate cotransporter), member 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N695
  • Protein Name
    Sodium-coupled monocarboxylate transporter 1
  • Scientific Description
    SLC5A8 has been shown to transport iodide by a passive mechanism (Rodriguez et al., 2002 [PubMed 12107270]) and monocarboxylates and short-chain fatty acids by a sodium-coupled mechanism (Gopal et al., 2004 [PubMed 15322102]). In kidney, SLC5A8 functions as a high-affinity sodium-coupled lactate transporter involved in reabsorption of lactate and maintenance of blood lactate levels (Thangaraju et al., 2006 [PubMed 16873376]).[supplied by OMIM, Dec 2008]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC5A8 using anti-SLC5A8 antibody (A03883-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A8 antigen affinity purified polyclonal antibody (Catalog # A03883-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A8 at approximately 67 kDa. The expected band size for SLC5A8 is at 67 kDa.
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