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ProductsBack/SLC6A1 / GAT-1 Antibody

SLC6A1 / GAT-1 Antibody

LS-C750075
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSLC6A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC6A1 / GAT-1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 67kDa, while the observed MW by Western blot was 67kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 67kDa, while the observed MW by Western blot was 67kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6529
  • Target name
    SLC6A1
  • Target description
    solute carrier family 6 member 1
  • Target synonyms
    GABA transporter 1; GABATHG; GABATR; GAT1; MAE; sodium- and chloride-dependent GABA transporter 1; solute carrier family 6 (neurotransmitter transporter), member 1; solute carrier family 6 (neurotransmitter transporter, GABA), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAT1/SLC6A1 using anti-GAT1/SLC6A1 antibody (A05109-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAT1/SLC6A1 antigen affinity purified polyclonal antibody (Catalog # A05109-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAT1/SLC6A1 at approximately 67 kDa. The expected band size for GAT1/SLC6A1 is at 67 kDa.
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