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Western Blot analysis of rat stomach cells using NTT4 Polyclonal Antibody
Western Blot analysis of rat stomach cells using NTT4 Polyclonal Antibody
Western Blot analysis of rat stomach cells using NTT4 Polyclonal Antibody

SLC6A17 Antibody

CSB-PA004745
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC6A17
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLC6A17 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID388662
  • Target name
    SLC6A17
  • Target description
    solute carrier family 6 member 17
  • Target synonyms
    MRT48, NTT4, sodium-dependent neutral amino acid transporter SLC6A17, neurotransmitter transporter 4, orphan sodium- and chloride-dependent neurotransmitter transporter NTT4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1V8
  • Protein Name
    Sodium-dependent neutral amino acid transporter SLC6A17
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC6A17 using anti-SLC6A17 antibody (A12725-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A17 antigen affinity purified polyclonal antibody (Catalog # A12725-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A17 at approximately 81 kDa. The expected band size for SLC6A17 is at 81 kDa.
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