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IHC-P analysis of human kidney tissue using GTX46755 SLC6A18 antibody at 4.0-8.0microg/ml.
IHC-P analysis of human kidney tissue using GTX46755 SLC6A18 antibody at 4.0-8.0microg/ml.
IHC-P analysis of human kidney tissue using GTX46755 SLC6A18 antibody at 4.0-8.0microg/ml.

SLC6A18 antibody, Internal

GTX46755
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSLC6A18
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Overview

  • Supplier
    GeneTex
  • Product Name
    SLC6A18 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. IHC-P: 2-10 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID348932
  • Target name
    SLC6A18
  • Target description
    solute carrier family 6 member 18
  • Target synonyms
    inactive sodium-dependent neutral amino acid transporter B(0)AT3; sodium- and chloride-dependent transporter XTRP2; sodium channel-like protein; sodium-dependent neutral amino acid transporter B(0)AT3; solute carrier family 6 (neurotransmitter transporter), member 18; solute carrier family 6 (neutral amino acid transporter), member 18; system B(0) neutral amino acid transporter AT3; Xtrp2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96N87
  • Protein Name
    Sodium-dependent neutral amino acid transporter B(0)AT3
  • Scientific Description
    The SLC6 family of proteins, which includes SLC6A18, act as specific transporters for neurotransmitters, amino acids, and osmolytes like betaine, taurine, and creatine. SLC6 proteins are sodium cotransporters that derive the energy for solute transport from the electrochemical gradient for sodium ions (Hoglund et al., 2005 [PubMed 16125675]).[supplied by OMIM, Apr 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC6A18 using anti-SLC6A18 antibody (A10812-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A18 antigen affinity purified polyclonal antibody (Catalog # A10812-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A18 at approximately 71 kDa. The expected band size for SLC6A18 is at 71 kDa.
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