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ProductsBack/SLC7A2 Antibody

SLC7A2 Antibody

LS-C749571
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC7A2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SLC7A2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 71kDa/75kDa/76kDa, while the observed MW by Western blot was 80kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 71kDa/75kDa/76kDa, while the observed MW by Western blot was 80kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6542
  • Target name
    SLC7A2
  • Target description
    solute carrier family 7 member 2
  • Target synonyms
    ATRC2, CAT-2A, CAT-2B, CAT2, HCAT2, SLC7A2A, SLC7A2B, cationic amino acid transporter 2, low affinity cationic amino acid transporter 2, solute carrier family 7 (cationic amino acid transporter, y+ system), member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CAT2/SLC7A2 using anti-CAT2/SLC7A2 antibody (A06193-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: rat ovary tissue lysates, Lane 4: mouse skeletal muscle tissue lysates, Lane 5: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAT2/SLC7A2 antigen affinity purified polyclonal antibody (Catalog # A06193-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAT2/SLC7A2 at approximately 100 kDa. The expected band size for CAT2/SLC7A2 is at 72 kDa.
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