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Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: SLFN11 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 103 kDa Observed band size: 103 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: SLFN11 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 103 kDa Observed band size: 103 kDa
Western Blot Positive WB detected in: Mouse skeletal muscle tissue All lanes: SLFN11 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 103 kDa Observed band size: 103 kDa

SLFN11 Antibody

CSB-PA021760LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSLFN11
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Overview

  • Supplier
    Cusabio
  • Product Name
    SLFN11 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID91607
  • Target name
    SLFN11
  • Target description
    schlafen family member 11
  • Target synonyms
    SLFN8/9, schlafen family member 11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z7L1
  • Protein Name
    Schlafen family member 11
  • Scientific Description
    Interferon (IFN)-induced antiviral protein which acts as an inhibitor of retrovirus protein synthesis. Specifically abrogates the production of retroviruses such as human immunodeficiency virus 1 (HIV-1) by acting as a specific inhibitor of the synthesis of retroviruses encoded proteins in a codon-usage-dependent manner. Binds to tRNAs and exploits the unique viral codon bias towards A/T nucleotides. The exact inhibition mechanism is unclear: may either sequesters tRNAs, prevents their maturation via post-transcriptional processing or accelerates their deacylation. Does not inhibit reverse transcription, integration or production and nuclear export of viral RNA. May play a role in cell cycle arrest and/or induction of apoptosis in response to exogenously induced DNA damage.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLFN11 using anti-SLFN11 antibody (A08259). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLFN11 antigen affinity purified polyclonal antibody (Catalog # A08259) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLFN11 at approximately 103 kDa. The expected band size for SLFN11 is at 103 kDa.
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