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ProductsBack/SMAD3 (phospho Ser423/Ser425) antibody [GT1207]
Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/Ser425) antibody [GT1207] (GTX00969) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/Ser425) antibody [GT1207] (GTX00969) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/Ser425) antibody [GT1207] (GTX00969) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SMAD3 (phospho Ser423/Ser425) antibody [GT1207]

GTX00969
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSMAD3
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Overview

  • Supplier
    GeneTex
  • Product Name
    SMAD3 (phospho Ser423/Ser425) antibody [GT1207]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1207
  • Conjugate
    Unconjugated
  • Gene ID4088
  • Target name
    SMAD3
  • Target description
    SMAD family member 3
  • Target synonyms
    HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3, mothers against decapentaplegic homolog 3, MAD homolog 3, MAD, mothers against decapentaplegic homolog 3, SMA- and MAD-related protein 3, SMAD, mothers against DPP homolog 3, mad homolog JV15-2, mad protein homolog, mothers against DPP homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP84022
  • Protein Name
    Mothers against decapentaplegic homolog 3
  • Scientific Description
    The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Pirfenidone suppressed triple-negative breast cancer metastasis by inhibiting the activity of the TGF-beta/SMAD pathway.
    Read this paper
  • Ameliorating role of microRNA-378 carried by umbilical cord mesenchymal stem cells-released extracellular vesicles in mesangial proliferative glomerulonephritis. Chen W et al., 2022 Mar 9, Cell Commun Signal
    Read this paper
  • Characterization of TGF-beta by Induced Oxidative Stress in Human Trabecular Meshwork Cells. Chen HY et al., 2021 Jan 13, Antioxidants (Basel)
    Read this paper
  • miR-18a Inhibits BMP4 and HIF-1alpha Normalizing Brain Arteriovenous Malformations. Marin-Ramos NI et al., 2020 Oct 9, Circ Res
    Read this paper

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