IHC-P analysis of rat brain tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207]. Dilution : 1:100
![IHC-P analysis of human esophageal tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00969/GTX00969_20200327_IHC-P_36_w_23053121_351.webp "IHC-P analysis of human esophageal tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207]. Dilution : 1:100")
![IHC-P analysis of mouse testis tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00969/GTX00969_20200327_IHC-P_37_w_23053121_739.webp "IHC-P analysis of mouse testis tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207]. Dilution : 1:100")
![WB analysis of C6 cell extracts with/without PMA/TPA (200 nM, 37oC, 10 min) treatment using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00969/GTX00969_20200327_WB_1_w_23053121_349.webp "WB analysis of C6 cell extracts with/without PMA/TPA (200 nM, 37oC, 10 min) treatment using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207]. Dilution : 1:1000 Loading : 25microg")
![Untreated (-) and treated (+) A549 whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/Ser425) antibody [GT1207] (GTX00969) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00969/GTX00969_4000000170_20200313_WB_treatment_TGF-beta1_w_23053121_737.webp "Untreated (-) and treated (+) A549 whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/Ser425) antibody [GT1207] (GTX00969) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
IHC-P analysis of rat brain tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207]. Dilution : 1:100
SMAD3 (phospho Ser423/Ser425) antibody [GT1207] - Recombinant antibody, Orthogonal Validated
GTX00969
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSMAD3
Overview
- SupplierGeneTex
- Product NameSMAD3 (phospho Ser423/Ser425) antibody [GT1207] - Recombinant antibody, Orthogonal Validated
- Delivery Days Customer9
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1207
- ConjugateUnconjugated
- Gene ID4088
- Target nameSMAD3
- Target descriptionSMAD family member 3
- Target synonymshMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; mad3; MADH3; mothers against decapentaplegic homolog 3; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionThe protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
- ReactivityHuman, Mouse, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Ameliorating role of microRNA-378 carried by umbilical cord mesenchymal stem cells-released extracellular vesicles in mesangial proliferative glomerulonephritis. Chen W et al., 2022 Mar 9, Cell Commun SignalRead more
- Characterization of TGF-beta by Induced Oxidative Stress in Human Trabecular Meshwork Cells. Chen HY et al., 2021 Jan 13, Antioxidants (Basel)Read more
- miR-18a Inhibits BMP4 and HIF-1alpha Normalizing Brain Arteriovenous Malformations. Marin-Ramos NI et al., 2020 Oct 9, Circ ResRead more