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Western Blot analysis of K562 cells using Smad5 Polyclonal Antibody
Western Blot analysis of K562 cells using Smad5 Polyclonal Antibody
Western Blot analysis of K562 cells using Smad5 Polyclonal Antibody

SMAD5 Antibody

CSB-PA004732
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetSMAD5
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Overview

  • Supplier
    Cusabio
  • Product Name
    SMAD5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4090
  • Target name
    SMAD5
  • Target description
    SMAD family member 5
  • Target synonyms
    DWFC; JV5-1; MAD, mothers against decapentaplegic homolog 5; MADH5; mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; SMA- and MAD-related protein 5; SMAD, mothers against DPP homolog 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99717
  • Protein Name
    Mothers against decapentaplegic homolog 5
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMAD5 using anti-SMAD5 antibody (A01423). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4:monkey COS-7 whole cell lysates, Lane 5: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD5 antigen affinity purified polyclonal antibody (Catalog # A01423) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD5 at approximately 52-60 kDa. The expected band size for SMAD5 is at 52 kDa.
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