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Immunohistochemistry of paraffin-embedded human appendix tissue using CSB-PA811625LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human appendix tissue using CSB-PA811625LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human appendix tissue using CSB-PA811625LA01HU at dilution of 1:100

SMAP1 Antibody

CSB-PA811625LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSMAP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SMAP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID60682
  • Target name
    SMAP1
  • Target description
    small ArfGAP 1
  • Target synonyms
    SMAP-1; stromal membrane-associated GTPase-activating protein 1; stromal membrane-associated protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IYB5
  • Protein Name
    Stromal membrane-associated protein 1
  • Scientific Description
    GTPase activating protein that acts on ARF6. Plays a role in clathrin-dependent endocytosis. May play a role in erythropoiesis (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMAP1 using anti-SMAP1 antibody (A11009). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAP1 antigen affinity purified polyclonal antibody (Catalog # A11009) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAP1 at approximately 60 kDa. The expected band size for SMAP1 is at 50 kDa.
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