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SMARCA2/BRM Recombinant Antibody

BSM-60621R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSMARCA2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    SMARCA2/BRM Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    E7F8
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6595
  • Target name
    SMARCA2
  • Target description
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
  • Target synonyms
    ATP-dependent helicase SMARCA2; BAF190; BAF190B; BIS; brahma homolog; BRG1-associated factor 190B; BRM; global transcription activator homologous sequence; hBRM; hSNF2a; NCBRS; probable global transcription activator SNF2L2; protein brahma homolog; SNF2; SNF2/SWI2-like protein 2; SNF2-alpha; SNF2L2; SNF2LA; Sth1p; sucrose nonfermenting 2-like protein 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2; SWI2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51531
  • Protein Name
    Probable global transcription activator SNF2L2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Flow Cytometry analysis of HeLa cells using anti-SMARCA2 antibody (A01888-2). Overlay histogram showing HeLa cells stained with A01888-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMARCA2 Antibody (A01888-2,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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