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SMARCA5 Antibody (YA2013)

HY-P82268
MedChem Express
TargetSMARCA5
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SMARCA5 Antibody (YA2013)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8467
  • Target name
    SMARCA5
  • Target description
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
  • Target synonyms
    hISWI; hSNF2H; ISWI; SNF2H; sucrose nonfermenting protein 2 homolog; sucrose nonfermenting-like 5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; WCRF135
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SMARCA5 Antibody (YA2013) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2013), targeting SMARCA5, with a predicted molecular weight of 122 kDa (observed band size: 122 kDa). SMARCA5 Antibody (YA2013) can be used for WB, ICC/IF experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNF2H/SMARCA5 antigen affinity purified polyclonal antibody (Catalog # A02687-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNF2H/SMARCA5 at approximately 122 kDa. The expected band size for SNF2H/SMARCA5 is at 122 kDa.
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