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SMARCA5 / SNF2H Antibody

LS-C331814
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
TargetSMARCA5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SMARCA5 / SNF2H Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 121kDa, while the observed MW by Western blot was 125kDa.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Applications Supplier
    ChIP-Seq (1:20 - 1:100), IF (1:50 - 1:200), IHC (1:50 - 1:200), IP (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 121kDa, while the observed MW by Western blot was 125kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.24 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8467
  • Target name
    SMARCA5
  • Target description
    SNF2 related chromatin remodeling ATPase 5
  • Target synonyms
    ISWI, SNF2H, WCRF135, hISWI, hSNF2H, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5, sucrose nonfermenting protein 2 homolog, sucrose nonfermenting-like 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNF2H/SMARCA5 using anti-SNF2H/SMARCA5 antibody (A02687-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNF2H/SMARCA5 antigen affinity purified polyclonal antibody (Catalog # A02687-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNF2H/SMARCA5 at approximately 122 kDa. The expected band size for SNF2H/SMARCA5 is at 122 kDa.
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