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ProductsBack/SMC1 (SMC1A) Mouse Monoclonal Antibody [Clone ID: 5B6]
Western blot analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.
Western blot analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.
Western blot analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.

SMC1 (SMC1A) Mouse Monoclonal Antibody [Clone ID: 5B6]

AM06475SU-N
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSMC1A
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SMC1 (SMC1A) Mouse Monoclonal Antibody [Clone ID: 5B6]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5B6
  • Gene ID8243
  • Target name
    SMC1A
  • Target description
    structural maintenance of chromosomes 1A
  • Target synonyms
    CDLS2, DEE85, DXS423E, EIEE85, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB, structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, epididymis secretory sperm binding protein, segregation of mitotic chromosomes 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ14683
  • Protein Name
    Structural maintenance of chromosomes protein 1A
  • Scientific Description
    SMC1 (SMC1A) mouse monoclonal antibody, clone 5B6, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMC1A using anti-SMC1A antibody (A02148-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC1A antigen affinity purified polyclonal antibody (Catalog # A02148-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC1A at approximately 150 kDa. The expected band size for SMC1A is at 143 kDa.
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