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ProductsBack/SMC1 (SMC1A) Mouse Monoclonal Antibody [Clone ID: C2M]
Figure 1. Western blotting using SMC1 antibody on HeLa cell lysate at 1 ug/ml.
Figure 1. Western blotting using SMC1 antibody on HeLa cell lysate at 1 ug/ml.
Figure 1. Western blotting using SMC1 antibody on HeLa cell lysate at 1 ug/ml.

SMC1 (SMC1A) Mouse Monoclonal Antibody [Clone ID: C2M]

AM05298PU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetSMC1A
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SMC1 (SMC1A) Mouse Monoclonal Antibody [Clone ID: C2M]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C2M
  • Gene ID8243
  • Target name
    SMC1A
  • Target description
    structural maintenance of chromosomes 1A
  • Target synonyms
    CDLS2, DEE85, DXS423E, EIEE85, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB, structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, epididymis secretory sperm binding protein, segregation of mitotic chromosomes 1
  • Host
    Mouse
  • Protein IDQ14683
  • Protein Name
    Structural maintenance of chromosomes protein 1A
  • Scientific Description
    SMC1 (SMC1A) mouse monoclonal antibody, clone C2M, Purified
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SMC1A using anti-SMC1A antibody (A02148-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC1A antigen affinity purified polyclonal antibody (Catalog # A02148-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC1A at approximately 150 kDa. The expected band size for SMC1A is at 143 kDa.
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