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SMC1B Antibody

LS-C747975
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetSMC1B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SMC1B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 135kDa/143kDa, while the observed MW by Western blot was 144kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) The predicted MW is 135kDa/143kDa, while the observed MW by Western blot was 144kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27127
  • Target name
    SMC1B
  • Target description
    structural maintenance of chromosomes 1B
  • Target synonyms
    SMC1BETA, SMC1L2, structural maintenance of chromosomes protein 1B, SMC protein 1B, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, SMC1 structural maintenance of chromosomes 1-like 2, mitosis-specific chromosome segregation protein like protein beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMC1B using anti-SMC1B antibody (A10280-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC1B antigen affinity purified polyclonal antibody (Catalog # A10280-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC1B at approximately 150,250 kDa. The expected band size for SMC1B is at 144 kDa.
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